An AIE‐active probe for efficient detection and high‐throughput identification of outer membrane vesicles

Rapid detection and quantification of outer membrane vesicle (OMV) are both scientific value clinical implications. However, limited tools available for investigations OMVs. Herein, we report a novel fluorescent probe with aggregation-induced emission (AIE) characteristics, namely, OEO-TPE-MEM (OTM), OMV detection. OTM emits faintly in an aqueous medium, but its fluorescence could be effectively turned on upon interacting bacteria bodies OMVs produced by Gram-negative bacteria. Notably, provide quantitative information bacterial remodeling secretion applied to high-throughput screening OMV-inducing agents. This study presents powerful AIE imaging analysis envelop derived OMVs, which might evaluating research antimicrobial materials future studies.